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nectin 2 pe  (Miltenyi Biotec)


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    Miltenyi Biotec nectin 2 pe
    Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and <t>Nectin-2,</t> respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).
    Nectin 2 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+human+cd112+antibody/CD112+Antibody%2C+anti-human%2C+REAfinity/pmc12901471-67-27-33
    Average 94 stars, based on 7 article reviews
    nectin 2 pe - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Osteoclasts affect the anti-cancer activity of NK cells"

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1730283

    Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and Nectin-2, respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).
    Figure Legend Snippet: Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and Nectin-2, respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Techniques Used: Expressing, Flow Cytometry

    Analysis of CD133 + /CXCR4 + CSC subset. Evaluation of the CSC CD133 + /CXCR4 + subset of A549s, in co-culture with NK cells, OCs and with both OCs and NK cells (A) . Evaluation of expression levels of Nectin-2 (ligand of TIGIT and DNAM-1) (B) and Nectin-4 (ligand of TIGIT) (C) on CD133 + cells, in co-culture with NK cells, OCs and with NK cells+OCs (B, C) . N = 5 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).
    Figure Legend Snippet: Analysis of CD133 + /CXCR4 + CSC subset. Evaluation of the CSC CD133 + /CXCR4 + subset of A549s, in co-culture with NK cells, OCs and with both OCs and NK cells (A) . Evaluation of expression levels of Nectin-2 (ligand of TIGIT and DNAM-1) (B) and Nectin-4 (ligand of TIGIT) (C) on CD133 + cells, in co-culture with NK cells, OCs and with NK cells+OCs (B, C) . N = 5 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Techniques Used: Co-Culture Assay, Expressing

    Related Articles

    Expressing:

    Article Title: Investigation of an oncolytic herpes simplex virus as a potential therapeutic agent for gastroenteropancreatic neuroendocrine neoplasms.
    Article Snippet: Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody. .. Briefly, phycoerythrin (PE) conjugated anti-human CD111 antibody (10 μL, Miltenyi Biotec), PE anti-human CD112 antibody (10 μL, Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (10 μL, Miltenyi Biotec), or APC anti-human HVEM (10 μL, Miltenyi Biotec), were added for 20 min on ice in the dark. .. Cells treated with FcR Scientific Reports | (2025) 15:13356 2| https://doi.org/10.1038/s41598-025-98588-7 blocker alone served a

    Article Title: Investigation of an oncolytic herpes simplex virus as a potential therapeutic agent for gastroenteropancreatic neuroendocrine neoplasms
    Article Snippet: FcR Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody.FcR Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody.. Briefly, phycoerythrin (PE) conjugated anti-human CD111 antibody (10 μL, Miltenyi Biotec), PE anti-human CD112 antibody (10 μL, Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (10 μL, Miltenyi Biotec), or APC anti-human HVEM (10 μL, Miltenyi Biotec), were added for 20 min on ice in the dark.. Cells treated with FcR blocker alone served as negative controls.Cells treated with FcR blocker alone served as negative controls.

    Article Title: Targeting High-Risk Neuroblastoma Patient-Derived Xenografts with Oncolytic Virotherapy.
    Article Snippet: only given blocker served as negative controls. .. After 15 min, 10 μL of phycoerythrin (PE) conjugated anti-human CD111 antibody (Miltenyi Biotec), PE anti-human CD112 antibody (Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (Miltenyi Biotec), or APC anti-human HVEM (Miltenyi Biotec), were added for 20 min on ice in the dark. .. The stained cells were centrifuged, washed twice with

    Article Title: Targeting High-Risk Neuroblastoma Patient-Derived Xenografts with Oncolytic Virotherapy
    Article Snippet: Cells only given blocker served as negative controls.Cells only given blocker served as negative controls.. After 15 min, 10 μL of phycoerythrin (PE) conjugated anti-human CD111 antibody (Miltenyi Biotec), PE anti-human CD112 antibody (Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (Miltenyi Biotec), or APC anti-human HVEM (Miltenyi Biotec), were added for 20 min on ice in the dark.. The stained cells were centrifuged, washed twice with autoMACS ® buffer (Miltenyi Biotec), and reconstituted in 200 μL of sterile phosphate buffered saline (PBS) for analysis via Attune Next Su™ Flow Cytometer (Thermo Fisher Scientific).The stained cells were centrifuged, washed twice with autoMACS ® buffer (Miltenyi Biotec), and reconstituted in 200 μL of sterile phosphate buffered saline (PBS) for analysis via Attune Next Su™ Flow Cytometer (Thermo Fisher Scientific).

    Flow Cytometry:

    Article Title: Investigation of an oncolytic herpes simplex virus as a potential therapeutic agent for gastroenteropancreatic neuroendocrine neoplasms.
    Article Snippet: Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody. .. Briefly, phycoerythrin (PE) conjugated anti-human CD111 antibody (10 μL, Miltenyi Biotec), PE anti-human CD112 antibody (10 μL, Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (10 μL, Miltenyi Biotec), or APC anti-human HVEM (10 μL, Miltenyi Biotec), were added for 20 min on ice in the dark. .. Cells treated with FcR Scientific Reports | (2025) 15:13356 2| https://doi.org/10.1038/s41598-025-98588-7 blocker alone served a

    Article Title: Investigation of an oncolytic herpes simplex virus as a potential therapeutic agent for gastroenteropancreatic neuroendocrine neoplasms
    Article Snippet: FcR Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody.FcR Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody.. Briefly, phycoerythrin (PE) conjugated anti-human CD111 antibody (10 μL, Miltenyi Biotec), PE anti-human CD112 antibody (10 μL, Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (10 μL, Miltenyi Biotec), or APC anti-human HVEM (10 μL, Miltenyi Biotec), were added for 20 min on ice in the dark.. Cells treated with FcR blocker alone served as negative controls.Cells treated with FcR blocker alone served as negative controls.

    Article Title: Targeting High-Risk Neuroblastoma Patient-Derived Xenografts with Oncolytic Virotherapy.
    Article Snippet: only given blocker served as negative controls. .. After 15 min, 10 μL of phycoerythrin (PE) conjugated anti-human CD111 antibody (Miltenyi Biotec), PE anti-human CD112 antibody (Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (Miltenyi Biotec), or APC anti-human HVEM (Miltenyi Biotec), were added for 20 min on ice in the dark. .. The stained cells were centrifuged, washed twice with

    Article Title: Targeting High-Risk Neuroblastoma Patient-Derived Xenografts with Oncolytic Virotherapy
    Article Snippet: Cells only given blocker served as negative controls.Cells only given blocker served as negative controls.. After 15 min, 10 μL of phycoerythrin (PE) conjugated anti-human CD111 antibody (Miltenyi Biotec), PE anti-human CD112 antibody (Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (Miltenyi Biotec), or APC anti-human HVEM (Miltenyi Biotec), were added for 20 min on ice in the dark.. The stained cells were centrifuged, washed twice with autoMACS ® buffer (Miltenyi Biotec), and reconstituted in 200 μL of sterile phosphate buffered saline (PBS) for analysis via Attune Next Su™ Flow Cytometer (Thermo Fisher Scientific).The stained cells were centrifuged, washed twice with autoMACS ® buffer (Miltenyi Biotec), and reconstituted in 200 μL of sterile phosphate buffered saline (PBS) for analysis via Attune Next Su™ Flow Cytometer (Thermo Fisher Scientific).

    Cell Culture:

    Article Title: Investigation of an oncolytic herpes simplex virus as a potential therapeutic agent for gastroenteropancreatic neuroendocrine neoplasms.
    Article Snippet: Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody. .. Briefly, phycoerythrin (PE) conjugated anti-human CD111 antibody (10 μL, Miltenyi Biotec), PE anti-human CD112 antibody (10 μL, Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (10 μL, Miltenyi Biotec), or APC anti-human HVEM (10 μL, Miltenyi Biotec), were added for 20 min on ice in the dark. .. Cells treated with FcR Scientific Reports | (2025) 15:13356 2| https://doi.org/10.1038/s41598-025-98588-7 blocker alone served a

    Article Title: Investigation of an oncolytic herpes simplex virus as a potential therapeutic agent for gastroenteropancreatic neuroendocrine neoplasms
    Article Snippet: FcR Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody.FcR Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody.. Briefly, phycoerythrin (PE) conjugated anti-human CD111 antibody (10 μL, Miltenyi Biotec), PE anti-human CD112 antibody (10 μL, Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (10 μL, Miltenyi Biotec), or APC anti-human HVEM (10 μL, Miltenyi Biotec), were added for 20 min on ice in the dark.. Cells treated with FcR blocker alone served as negative controls.Cells treated with FcR blocker alone served as negative controls.

    Article Title: Targeting High-Risk Neuroblastoma Patient-Derived Xenografts with Oncolytic Virotherapy.
    Article Snippet: only given blocker served as negative controls. .. After 15 min, 10 μL of phycoerythrin (PE) conjugated anti-human CD111 antibody (Miltenyi Biotec), PE anti-human CD112 antibody (Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (Miltenyi Biotec), or APC anti-human HVEM (Miltenyi Biotec), were added for 20 min on ice in the dark. .. The stained cells were centrifuged, washed twice with

    Article Title: Targeting High-Risk Neuroblastoma Patient-Derived Xenografts with Oncolytic Virotherapy
    Article Snippet: Cells only given blocker served as negative controls.Cells only given blocker served as negative controls.. After 15 min, 10 μL of phycoerythrin (PE) conjugated anti-human CD111 antibody (Miltenyi Biotec), PE anti-human CD112 antibody (Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (Miltenyi Biotec), or APC anti-human HVEM (Miltenyi Biotec), were added for 20 min on ice in the dark.. The stained cells were centrifuged, washed twice with autoMACS ® buffer (Miltenyi Biotec), and reconstituted in 200 μL of sterile phosphate buffered saline (PBS) for analysis via Attune Next Su™ Flow Cytometer (Thermo Fisher Scientific).The stained cells were centrifuged, washed twice with autoMACS ® buffer (Miltenyi Biotec), and reconstituted in 200 μL of sterile phosphate buffered saline (PBS) for analysis via Attune Next Su™ Flow Cytometer (Thermo Fisher Scientific).

    Staining:

    Article Title: Investigation of an oncolytic herpes simplex virus as a potential therapeutic agent for gastroenteropancreatic neuroendocrine neoplasms.
    Article Snippet: Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody. .. Briefly, phycoerythrin (PE) conjugated anti-human CD111 antibody (10 μL, Miltenyi Biotec), PE anti-human CD112 antibody (10 μL, Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (10 μL, Miltenyi Biotec), or APC anti-human HVEM (10 μL, Miltenyi Biotec), were added for 20 min on ice in the dark. .. Cells treated with FcR Scientific Reports | (2025) 15:13356 2| https://doi.org/10.1038/s41598-025-98588-7 blocker alone served a

    Article Title: Investigation of an oncolytic herpes simplex virus as a potential therapeutic agent for gastroenteropancreatic neuroendocrine neoplasms
    Article Snippet: FcR Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody.FcR Blocker (20 μL, 120-000-442, Miltenyi Biotec) was added, the suspensions rested on ice for 15 min and stained with antibody.. Briefly, phycoerythrin (PE) conjugated anti-human CD111 antibody (10 μL, Miltenyi Biotec), PE anti-human CD112 antibody (10 μL, Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (10 μL, Miltenyi Biotec), or APC anti-human HVEM (10 μL, Miltenyi Biotec), were added for 20 min on ice in the dark.. Cells treated with FcR blocker alone served as negative controls.Cells treated with FcR blocker alone served as negative controls.

    Article Title: Targeting High-Risk Neuroblastoma Patient-Derived Xenografts with Oncolytic Virotherapy.
    Article Snippet: only given blocker served as negative controls. .. After 15 min, 10 μL of phycoerythrin (PE) conjugated anti-human CD111 antibody (Miltenyi Biotec), PE anti-human CD112 antibody (Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (Miltenyi Biotec), or APC anti-human HVEM (Miltenyi Biotec), were added for 20 min on ice in the dark. .. The stained cells were centrifuged, washed twice with

    Article Title: Targeting High-Risk Neuroblastoma Patient-Derived Xenografts with Oncolytic Virotherapy
    Article Snippet: Cells only given blocker served as negative controls.Cells only given blocker served as negative controls.. After 15 min, 10 μL of phycoerythrin (PE) conjugated anti-human CD111 antibody (Miltenyi Biotec), PE anti-human CD112 antibody (Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (Miltenyi Biotec), or APC anti-human HVEM (Miltenyi Biotec), were added for 20 min on ice in the dark.. The stained cells were centrifuged, washed twice with autoMACS ® buffer (Miltenyi Biotec), and reconstituted in 200 μL of sterile phosphate buffered saline (PBS) for analysis via Attune Next Su™ Flow Cytometer (Thermo Fisher Scientific).The stained cells were centrifuged, washed twice with autoMACS ® buffer (Miltenyi Biotec), and reconstituted in 200 μL of sterile phosphate buffered saline (PBS) for analysis via Attune Next Su™ Flow Cytometer (Thermo Fisher Scientific).



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    Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and Nectin-2, respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

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    Article Title: Osteoclasts affect the anti-cancer activity of NK cells

    doi: 10.3389/fimmu.2026.1730283

    Figure Lengend Snippet: Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and Nectin-2, respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Article Snippet: After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA).

    Techniques: Expressing, Flow Cytometry

    Analysis of CD133 + /CXCR4 + CSC subset. Evaluation of the CSC CD133 + /CXCR4 + subset of A549s, in co-culture with NK cells, OCs and with both OCs and NK cells (A) . Evaluation of expression levels of Nectin-2 (ligand of TIGIT and DNAM-1) (B) and Nectin-4 (ligand of TIGIT) (C) on CD133 + cells, in co-culture with NK cells, OCs and with NK cells+OCs (B, C) . N = 5 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Journal: Frontiers in Immunology

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells

    doi: 10.3389/fimmu.2026.1730283

    Figure Lengend Snippet: Analysis of CD133 + /CXCR4 + CSC subset. Evaluation of the CSC CD133 + /CXCR4 + subset of A549s, in co-culture with NK cells, OCs and with both OCs and NK cells (A) . Evaluation of expression levels of Nectin-2 (ligand of TIGIT and DNAM-1) (B) and Nectin-4 (ligand of TIGIT) (C) on CD133 + cells, in co-culture with NK cells, OCs and with NK cells+OCs (B, C) . N = 5 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Article Snippet: After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA).

    Techniques: Co-Culture Assay, Expressing

    Immunophenotyping panel for multiplexed tissue imaging of cancer.

    Journal: Frontiers in Immunology

    Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

    doi: 10.3389/fimmu.2024.1383932

    Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

    Article Snippet: CD112 , REA1195 , 50 , 130-122-770 , PE , Miltenyi Biotec.

    Techniques: Imaging